Evaluation of a Fully Mechanized Enzymatic Kinetic Determination of Sialic Acid
نویسندگان
چکیده
منابع مشابه
New enzymatic determination of sialic acid in serum.
This enzymatic method for determination of sialic acid involves use of neuraminidase (EC 3.2.1.18), N-acetylneuraminate lyase (EC 4.1.3.3), acylglucosamine 2-epimerase (EC 5.1.3.8), N-acetylhexosamine oxidase (from Pseudomonas sp.), and peroxidase (EC 1.11.1.7). Because the method does not require pyruvic acid in the assay medium, interference by pyruvic acid in serum can be avoided. This simpl...
متن کاملhistological evaluation of the effect of three medicaments; trichloracetic acid, formocresol and mineral trioxide aggregate on pulpotomized teeth of dogs
چکیده ندارد.
15 صفحه اولEnzymatic synthesis of sialic acid 9-phosphates.
7Jourdian, G. W., and S. Roseman, Abstracts, 137th Meeting of the American Chemical Society, 1960, p. 47C. 8 Byrne, W. L., and H. A. Lardy, Biochim. et Biophys. Acta, 14, 495-601 (1954). 9 Dische, Z., and Borenfreund, E., J. Biol. Chem., 180, 1297-1300 (1949). 10 Ballou, C. E., Arch. Biochem. & Biophys., 78, 328-333 (1958). 11 Warren, L., and H. Felsenfeld, Biochem. Biophys. Res. Comm., 4, 232-...
متن کاملKINETIC DETERMINATION OF ASCORBIC ACID a WITH SPECTROPHOTOMETRIC DETECTION
A simple and rapid method has been developed for the determination of ascorbic acid in fruit and pharmaceutical products. The method is based on the measurement of the reaction rate between ammonium molybdate and ascorbic acid in acidic media. The reaction was monitored spectrophotometrically at 800 nm by fixed-time and variable-time methods. The calibration graph was linear in the range of...
متن کاملPlasma Sialic Acid Determination
The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucose tolerance using the enzymatic method were 1.747 ± 0.047 and 2.583 ± 0.070 mmole/l and 1.753 ± 0...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1985
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1985.23.11.781